Goat anti-Pig IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN101879

Supplier Product No.: 614-1302

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Pig

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Supplier Product No.: 614-1302

Supplier: Rockland

Purpose: Swine IgG (H&L) Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Swine IgG and Swine Serum.

Characteristics: Anti-Swine IgG (H&L) Fluorescein Antibody generated in goat detects reactivity to swine IgG.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Anti-Swine IgG whole molecule was produced by repeated immunization with Swine IgG whole molecule in goat.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Swine IgG Peroxidase conjugated has been tested by ELISA and western blot and is assayed against 1.0 μg of Swine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:5,000 to 1:20,000 of the reconstitution concentration is suggested for this product.  Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:100,000 - 1:200,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Anti-Swine IgG whole molecule antibody generated in goat detects specifically Swine IgG whole molecule. This secondary antibody anti-Rat is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.