Rabbit anti-Pig IgG (F(ab')2 Region) Antibody

Catalog No. ABIN101895

Supplier Product No.: 614-4104

Quick Overview for Rabbit anti-Pig IgG (F(ab')2 Region) Antibody (ABIN101895)

Target: IgG

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: F(ab')2 Region

Reactivity: Pig

Host: Rabbit

Supplier Product No.: 614-4104

Supplier: Rockland

Purpose: Swine IgG F(ab')2 Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Swine IgG, Swine IgG F(ab')2 and Swine Serum.  No reaction was observed against Swine IgG F(c).

Characteristics: SWINE IgG (H&L) (RABBIT) Antibody generated in rabbit detects specifically swine IgG whole molecule. Anti-Swine IgG antibody is ideal for investigators involved in serum protein component research.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Swine IgG F(ab')2 fragment

Application Details

Application Notes: Application Note: Anti-Swine IgG F(ab')2 antibody has been tested by ELISA and is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 4.6 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: Anti-Swine IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the Swine IgG molecule. Swine IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Swine IgG F(c) will not bind Swine IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.