Rat anti-Rabbit IgG (Heavy & Light Chain) Antibody - Preadsorbed

Catalog No. ABIN101924

Supplier Product No.: 611-501-122

Quick Overview for Rat anti-Rabbit IgG (Heavy & Light Chain) Antibody - Preadsorbed (ABIN101924)

Target: IgG

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Rat

Supplier Product No.: 611-501-122

Supplier: Rockland

Purpose: Rabbit IgG (H&L) Antibody Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Hu, Gt, Ms Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Goat, Human and Mouse Serum Proteins.

Characteristics: Anti-Rabbit IgG Antibody Rhodamine generated in rat detects rabbit IgG.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Rabbit IgG whole molecule

Application Details

Application Notes: Application Note: Anti-Rabbit IgG antibody has been tested by ELISA and western blot and is suitable for use in immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:20,000 - 1:100,000 ELISA Dilution: 1:900,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: Anti-Rabbit IgG (H&L) generated in rat detects rabbit Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.