Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody - Preadsorbed

Catalog No. ABIN101951

Supplier Product No.: 611-701-127

Quick Overview for Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody - Preadsorbed (ABIN101951)

Target: IgG

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Donkey

Supplier Product No.: 611-701-127

Supplier: Rockland

Specificity: IgG (H&L)

Characteristics: Anti-Rabbit IgG antibody generated in donkey detects specifically rabbit IgG. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Sterility: Sterile filtered

Immunogen: Anti-Rabbit IgG was produced by repeated immunization with rabbit whole IgG molecule in donkey.

Application Details

Application Notes: Anti-Rabbit IgG (H&l) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C

References

He, Qiu, Dai, Fang, Sun, Gong, Ding, Sun: "MicroRNA-351 inhibits denervation-induced muscle atrophy by targeting TRAF6." in: Experimental and therapeutic medicine, Vol. 12, Issue 6, pp. 4029-4034, (2016) (PubMed).

Hua, Han, Zhou, Kim, Shi, Abeysirigunawardena, Jain, Singh, Aggarwal, Woodson, Ha: "An improved surface passivation method for single-molecule studies." in: Nature methods, Vol. 11, Issue 12, pp. 1233-6, (2015) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Synonyms: Rabbit Antibody, Dk-a-Rabbit IgG, Rabbit Antibody in Donkey, Rabbit Secondary Antibody.