Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed

Catalog No. ABIN101954

Supplier Product No.: 611-702-127

Quick Overview for Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed (ABIN101954)

Target: IgG

Clonality: Polyclonal

Conjugate: FITC

Application: Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Donkey

Supplier Product No.: 611-702-127

Supplier: Rockland

Purpose: Rabbit IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Rabbit IgG, and Rabbit Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

Characteristics: Anti-Rabbit IgG F(c) fluorescein antibody generated in donkey is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Rabbit IgG whole molecule

Application Details

Application Notes: Application Note: Anti-Rabbit IgG (H&L) Fluorescein Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

Tseng, Wang, Lee, Bowen, Gicking, Guo, Liu, Qiu: "The preprophase band-associated kinesin-14 OsKCH2 is a processive minus-end-directed microtubule motor." in: Nature communications, Vol. 9, Issue 1, pp. 1067, (2018) (PubMed).

Hughes, Gândara, Javkar, Sablitzky, Hock, Potten, Mahida: "Heterogeneity in histone 2B-green fluorescent protein-retaining putative small intestinal stem cells at cell position 4 and their absence in the colon." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 303, Issue 11, pp. G1188-201, (2013) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.