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Donkey anti-Rabbit IgG (Fc Region) Antibody

ELISA, IHC, WB Polyclonal unconjugated
Rockland
Catalog No. ABIN102057
Supplier Product No.: 111-7103
  • Target See all IgG products
    IgG
    Binding Specificity
    • 2398
    • 514
    • 417
    • 146
    • 129
    • 128
    • 67
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 1
    • 1
    Fc Region
    Reactivity
    • 730
    • 693
    • 595
    • 462
    • 399
    • 240
    • 208
    • 175
    • 145
    • 137
    • 112
    • 107
    • 106
    • 101
    • 82
    • 58
    • 47
    • 28
    • 27
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Rabbit
    Host
    • 1762
    • 1547
    • 566
    • 258
    • 112
    • 111
    • 32
    • 31
    • 21
    • 18
    • 15
    • 11
    • 11
    Donkey
    Clonality
    • 3390
    • 179
    • 16
    • 1
    Polyclonal
    Application
    • 2058
    • 1984
    • 1850
    • 1804
    • 998
    • 920
    • 896
    • 487
    • 424
    • 234
    • 234
    • 149
    • 98
    • 84
    • 65
    • 56
    • 51
    • 30
    • 28
    • 25
    • 20
    • 15
    • 11
    • 9
    • 8
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Supplier Product No.
    111-7103
    Supplier
    Rockland
    Purpose
    Rabbit IgG Fc Antibody
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-donkey serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum.  No reaction was observed against Rabbit IgG F(ab).
    Characteristics
    Anti-Rabbit IgM antibody generated in goat specifically detects rabbit IgM mu heavy chain.
    Purification
    This product was prepared from monospecific antiserum by a delipidation and defibrination.
    Immunogen

    Immunogen: Rabbit IgG F(c) fragment

    Immunogen Type: Native Protein

  • Application Notes
    Application Note: Antibody Anti-Rabbit IgG F(c) is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
    Concentration
    80 mg/mL
    Buffer

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    , Preservative:None
    Preservative
    Without preservative
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background
    Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
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