Chicken anti-Rat IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN102091

Supplier Product No.: 612-9302

Quick Overview for Chicken anti-Rat IgG (Heavy & Light Chain) Antibody (HRP) (ABIN102091)

Target: IgG

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Rat

Host: Chicken

Supplier Product No.: 612-9302

Supplier: Rockland

Purpose: Rat IgG (H&L) Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Chicken Serum, Rat IgG and Rat Serum.

Characteristics: Anti-Rat IgG (H&L) Fluorescein Antibody generated in chicken detects reactivity to Rat IgG.

Purification: Anti-RAT IgG antibody, Peroxidase Conjugate, was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose.

Immunogen: Optional[Immunogen]: Rat IgG whole molecule

Application Details

Application Notes: Application Note: Anti-RAT IgG Peroxidase Conjugated antibody has been tested by ELISA and is assayed against 1.0 μg of Rat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid] substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:12,000 of the reconstitution concentration is suggested for this product.  Optimal concentration in other immunoassays should be determined by researcher. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:10,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: RAT IgG Antibody, Peroxidase Conjugated, is specific for the heavy and light chains of all subclasses of Rat immunoglulin G. Rat immunoglobulin G can be categorized into 4 IgG sub classes: IgG1, IgG2a, IgG2b, IgG2c.