Rabbit anti-Rat IgG (Heavy & Light Chain) Antibody

Catalog No. ABIN102162

Supplier Product No.: 612-4126

Quick Overview for Rabbit anti-Rat IgG (Heavy & Light Chain) Antibody (ABIN102162)

Target: IgG

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Rat

Host: Rabbit

Supplier Product No.: 612-4126

Supplier: Rockland

Purpose: Rat IgG (H&L) Antibody Pre-adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Rat IgG and Rat Serum. No reaction was observed against Human Serum Proteins.

Characteristics: Anti-Rat IgM antibody generated in rabbit specifically detects rat IgM heavy chain.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Anti-Rat IgG (H&L) was produced by repeated immunization with rat IgG whole molecule in rabbit

Application Details

Application Notes: Application Note: Anti-Rat IgG has been tested by ELISA and western blot and has been assayed against 1.0 μg of Rat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:1,000-1:5,000 Western Blot Dilution: 1:2,000-1:10,000 ELISA Dilution: 1:20,000 - 1:300,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.2 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: Anti-Rat IgG (H&L) antibody generated in rabbit detects specifically rat IgG (H&L). This secondary antibody anti-Rat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.