Donkey anti-Sheep IgG (Heavy & Light Chain) Antibody

Catalog No. ABIN102219

Supplier Product No.: 613-7102

Quick Overview for Donkey anti-Sheep IgG (Heavy & Light Chain) Antibody (ABIN102219)

Target: IgG

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Sheep

Host: Donkey

Supplier Product No.: 613-7102

Supplier: Rockland

Purpose: Sheep IgG (H&L) Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Sheep IgG and Sheep Serum.

Characteristics: Anti-Sheep IgG (H&L) Texas Red™ Antibody generated in donkey detects reactivity to sheep IgG.

Purification: Anti-SHEEP IgG Antibody was prepared from monospecific antiserum generated in donkey by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Sheep IgG whole molecule

Application Details

Application Notes: Application Note: Anti-Sheep IgG secondary antibody has been tested by ELISA and is suitable for use in various immunoassays. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: Sheep IgG whole molecule antibody generated in donkey detects specifically Sheep IgG whole molecule. This secondary antibody anti-Sheep is ideal for investigators who routinely perform western blot, immunoprecipitation and more generally immunoassays.