Donkey anti-Sheep IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN102221

Supplier Product No.: 613-7502

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Sheep

Host: Donkey

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Supplier Product No.: 613-7502

Supplier: Rockland

Purpose: Sheep IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Sheep IgG and Sheep Serum.

Characteristics: Anti-Sheep IgG antibody generated in donkey detects specifically sheep IgG. This secondary antibody anti-Sheep is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Purification: Anti-Sheep IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Sheep IgG (H&L) was produced by repeated immunization with Sheep IgG whole molecule in donkey.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Sheep IgG Alkaline Phosphatase Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:1,000 - 1:4,000 ELISA Dilution: 1:2,000 - 1:8,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Anti-Sheep IgG (H&L) antibody generated in donkey detects specifically sheep IgG heavy and light chains (H&L). This Anti sheep antibody is conjugated to alkaline phosphatase and this secondary antibody is suitable for immunoblotting and immunoassays. Anti-Sheep IgG (H&L) antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.