Donkey anti-Sheep IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN102227

Supplier Product No.: 613-7302

Quick Overview for Donkey anti-Sheep IgG (Heavy & Light Chain) Antibody (HRP) (ABIN102227)

Target: IgG

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Sheep

Host: Donkey

Supplier Product No.: 613-7302

Supplier: Rockland

Purpose: Sheep IgG (H&L) Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Sheep IgG and Sheep Serum.

Characteristics: Anti-Sheep IgG (H&L) fluorescein Antibody generated in donkey detects reactivity to sheep IgG.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads.

Immunogen:

Immunogen: Anti-Sheep IgG (H&L) was produced by repeated immunization with Sheep IgG whole molecule in donkey.

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Sheep IgG Peroxidase conjugated antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:3,000 - 1:15,000 ELISA Dilution: 1:50,000 - 1:150,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: Anti-Sheep IgG antibody generated in donkey detects specifically sheep IgG. This secondary antibody anti-Sheep is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.