Rabbit anti-Mouse IgG2b Antibody (HRP)

Catalog No. ABIN102401

Supplier Product No.: 610-4342

Quick Overview for Rabbit anti-Mouse IgG2b Antibody (HRP) (ABIN102401)

Target: IgG2b

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Rabbit

Supplier Product No.: 610-4342

Supplier: Rockland

Purpose: Mouse IgG2b (Gamma 2b chain) Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.  Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other mouse or human heavy or light chain isotypes.

Characteristics: Anti-Mouse IgG2a heavy chain antibody generated in rabbit detects specifically Mouse IgG2a heavy chain. This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Anti-Mouse IgG2b was produced by repeated immunization with mouse IgG2b heavy chain in rabbit.

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Mouse IgG2b peroxidase conjugated antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:65,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Krieger, Stifter, Riedl, Schirmbeck: "Cationic domains in particle-forming and assembly-deficient HBV core antigens capture mammalian RNA that stimulates Th1-biased antibody responses by DNA vaccination." in: Scientific reports, Vol. 8, Issue 1, pp. 14660, (2018) (PubMed).

Target Details

Target: IgG2b

Target Type: Antibody

Background: Anti-Mouse IgG2b peroxidase conjugated antibody generated in rabbit detects specifically mouse IgG2b. This secondary peroxidase conjugated antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.