Goat anti-Human Ig (Chain kappa), (Free & Bound) Antibody - Preadsorbed
Quick Overview for Goat anti-Human Ig (Chain kappa), (Free & Bound) Antibody - Preadsorbed (ABIN1099686)
Target
Clonality
Application
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Binding Specificity
- Chain kappa, Free & Bound
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Reactivity
- Human
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Host
- Goat
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Characteristics
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To detect and identify the light chain type of immunoglobulins or free light chains in serum or other body fluids by immunoelectrophoresis or double radial immuno- diffusion.
Physical form: Delipidated, heat inactivated lyophilized whole serum. -
Purification
- Preadsorption: Immunoaffinity adsorbed using insolubilized antigens
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Immunogen
- A pool of purified Bence Jones kappa proteins isolated from human urine. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Application Notes
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Directions for use: In immunoelectrophoresis use 2 µL serum or equivalent against 120 µL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µL antiserum in 3 mm diameter center well and 2 µL serum samples (neat and serially diluted in 2 mm diameter peripheral wells. O
ptimal working dilutions should be determined experimentally by the investigator. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Preservative
- Without preservative
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Storage
- 4 °C/-20 °C
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Storage Comment
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- Ig
Target
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