Mouse anti-Human IgAc, IgA1, IgA2 (Secretory Component) Antibody

Catalog No. ABIN1099693

Quick Overview for Mouse anti-Human IgAc, IgA1, IgA2 (Secretory Component) Antibody (ABIN1099693)

Target: IgAc, IgA1, IgA2

Clonality: Polyclonal

Application: Immunofluorescence (IF), ELISA

Product Details

Binding Specificity: Secretory Component

Reactivity: Human

Host: Mouse

Characteristics: IgAc, IgA1, IgA2 SC (Class Specific)T
he reactivity of the antibody is restricted to the given protein as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic immunoglobulins.

Monoclonal antibody concentration in the solution is 1.0 mg/mL.

Physical form: Delipidated, heat inactivated, lyophilized stable ascites.

Purification: Ascites

Immunogen: Highly purified monoclonal IgA subclasses isolated from pooled human serum, secretory components isolated from human milk.

Application Details

Application Notes: The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly form 1:100 to 1:500, in ELISA from 1:1000 upwards, in Western blotting 1:2,000 to 1:10,000. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized ascites by adding 0.5 mL sterile distilled water.

Buffer: No foreign proteins added.

Handling Advice: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation. This will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgAc, IgA1, IgA2

Background: To identify the presence of IgA and secretory subclasses in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgA, subclasses or secretory component and immunoblotting.