Goat anti-Rat IgG2a Antibody (HRP)

Catalog No. ABIN1099728

Quick Overview for Goat anti-Rat IgG2a Antibody (HRP) (ABIN1099728)

Target: IgG2a

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Dot Blot (DB)

Product Details

Reactivity: Rat

Host: Goat

Characteristics: subclass specific

Physicochemical characteristics: IgG protein concentration 10 mg/mL. Peroxidase/IgG protein molar ratio (E/P) is approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2)

Physical form: Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Conjugation procedure: Conjugation is carried out using a proprietary modification of the periodate technique for the binding to peroxidase, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life.

Purification: Hyperimmune antisera with strong precipitating activity are selected for fractionation and purification of the IgG (7S) fraction containing the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis.
Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Pools of purified homogenous IgG2a isolated from rat sera. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000 depending on the method used.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Phosphate buffered saline (PBS, pH 7.2)

Preservative: Without preservative

Handling Advice: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +0 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgG2a

Target Type: Antibody

Background: In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG2a at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to demonstrate circulating IgG2a antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2a isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e. g. ELISA) to measure IgG2a in rat serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.