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Goat anti-Llama IgG (Heavy & Light Chain) Antibody (DyLight 550)

IA Polyclonal DyLight 550
Catalog No. ABIN1110431

Quick Overview for Goat anti-Llama IgG (Heavy & Light Chain) Antibody (DyLight 550) (ABIN1110431)

Target

IgG

Clonality

  • 3437
  • 230
  • 16
  • 1
Polyclonal

Conjugate

  • 665
  • 422
  • 422
  • 398
  • 274
  • 262
  • 235
  • 118
  • 105
  • 72
  • 67
  • 63
  • 61
  • 58
  • 58
  • 50
  • 44
  • 43
  • 40
  • 39
  • 39
  • 37
  • 35
  • 35
  • 32
  • 31
  • 31
  • 30
  • 29
  • 29
  • 29
  • 29
  • 29
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 27
  • 26
  • 26
  • 25
  • 21
  • 21
  • 19
  • 17
  • 17
  • 16
DyLight 550

Application

  • 2119
  • 2050
  • 1885
  • 1873
  • 999
  • 920
  • 896
  • 520
  • 424
  • 234
  • 234
  • 148
  • 102
  • 98
  • 88
  • 84
  • 51
  • 33
  • 30
  • 28
  • 25
  • 20
  • 15
  • 11
  • 9
  • 8
  • 7
  • 5
  • 5
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Immunoassay (IA)
  • Binding Specificity

    • 2396
    • 517
    • 417
    • 146
    • 130
    • 128
    • 66
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Heavy & Light Chain

    Reactivity

    • 753
    • 703
    • 607
    • 468
    • 410
    • 244
    • 213
    • 176
    • 146
    • 141
    • 124
    • 110
    • 107
    • 101
    • 82
    • 62
    • 47
    • 27
    • 27
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Llama

    Host

    • 1772
    • 1583
    • 567
    • 282
    • 122
    • 112
    • 41
    • 32
    • 22
    • 21
    • 16
    • 11
    • 11
    Goat

    Specificity

    Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on llama IgG and light chains on all llama immunoglobulins.

    Characteristics

    Goat anti-llama IgG (H&L) - DyLight® 550 Conjugate is a secondary antibody conjugated to DyLight® 550, which binds to all llama IgG in immunological assays. DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase llama IgG (H&L).

    Purification

    Affinity purification

    Immunogen

    Purified llama IgG, whole molecule
  • Application Notes

    1:20 - 1:2,000 for most applications

    Comment

    Based on immunoelectrophoresis, this antibody reacts with: heavy (gamma) chains on llama IgG light chains on all llama immunoglobulins.This antibody will react with VHH of llama IgG's.No reactivity is observed to: non-immunoglobulin llama serum proteins.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

    Handling Advice

    Product is photosensitive and should be protected from light.
    Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
    A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
  • Target

    IgG

    Target Type

    Antibody
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