Mouse anti-Human IgA Antibody

Catalog No. ABIN111961

Product Details

Target: IgA

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Affinity Chromatography

Clone: DA43D3

Isotype: IgG1

Application Details

Application Notes: Suitable for Immunohistochemistry on Frozen (1 μg/mL, 1/400) and Paraffin EmbeddedSections. Proteinase K pretreatment for antigen retrieval is recommended. Suggested Positive Control: Human tonsil.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with frozen, ice-cold acetone-fixed sections:
The whole procedure is performed at room temperature

1. Wash in PBS
2. Block endogenous peroxidase
3. Wash in PBS4. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber
5. Incubate with primary antibody (dilution see datasheet) for 1 h in a humid chamber
6. Wash in PBS
7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1 h in a humid chamber
8. Wash in PBS
9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
10. Wash in PBS
11. Counterstain with Mayer's hemalum.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: PBS, pH 7.2 with 0.01 % Thimerosal as preservative and 10 mg/mL BSA as stabilizer.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: Biohazard Informations: This product contains thimerosal which is hazardous.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Target Details

Target: IgA

Abstract: IgA Products

Target Type: Antibody

Background: Synonyms: Human Immunoglobulin A