Mouse anti-Human Ig (Chain lambda), (Light Chain) Antibody

Catalog No. ABIN114486

Quick Overview for Mouse anti-Human Ig (Chain lambda), (Light Chain) Antibody (ABIN114486)

Target: Ig

Clonality: Monoclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: N10-2

Isotype: IgG1

Product Details

Binding Specificity: Chain lambda, Light Chain

Reactivity: Human

Host: Mouse

Purification: Supernatant

Immunogen: BALB/C mice were immunized with purified IgG from human serum.

Application Details

Application Notes: Immunohistochemistry on paraffin embedded sections (1/25-1/75 with ABC method,incubate 30 minutes at room temperature, Enzymatic predigestion may improveimmunostaining in some cases). Positive Control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: Ig

Alternative Name: Immunoglobulin

Background: All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5 kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L, Lambda, 0.4-4.2 mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).