Goat anti-Ferret IgG (Chain gamma) Antibody (FITC) - Preadsorbed
WB, FLISA, IM
Polyclonal
FITC
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- Target See all IgG products
- IgG
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Binding Specificity
- Chain gamma
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Reactivity
- Ferret
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- FITC
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Application
- Western Blotting (WB), FLISA, Immunomicroscopy (IM)
- Characteristics
- Molar Ratio: 4.0 moles FITC per mole of Goat IgG.
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
- Ferret IgG gamma gamma chain.
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- Application Notes
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This product is designed for Immunofluorescence Microscopy, fluorescence based plateassays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplexanalysis, including multicolor imaging, utilizing various commercial platforms. Recommended DilutionsFLISA: 1/10,000-1/50,000. IF Microscopy: 1/1,000-1/5,000. Flow Cytometry: 1/500-1/2,500.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - Comment
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Absorption / Emission: 495 nm / 528 nm
- Restrictions
- For Research Use only
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- Reconstitution
- Restore with 1.0 mL of deionized water (or equivalent).
- Concentration
- 1.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
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Product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Synonyms: Ferret Immunoglobulin G
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