Chicken anti-Human IgG (Whole Molecule) Antibody (HRP) - Preadsorbed
Quick Overview for Chicken anti-Human IgG (Whole Molecule) Antibody (HRP) - Preadsorbed (ABIN117235)
Target
Clonality
Conjugate
Application
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Binding Specificity
- Whole Molecule
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Reactivity
- Human
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Host
- Chicken
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Purification
- Preadsorption: Solid phase absorption
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Immunogen
- Human IgG whole molecule
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Mouse anti-Human IgG (Fc Region) antibody
Animal-Free ELISA, FACS, WB, IF Multiclonal IgG2a Recombinant Antibody unconjugated
Mouse anti-Human IgG (Fc Region) antibody (HRP)Animal-Free ELISA, FACS, WB, IF Multiclonal IgG2a Recombinant Antibody HRP
Mouse anti-Human IgG (Fc Region) antibody (APC)ELISA, FACS Monoclonal H2 IgG2b APC
Goat anti-Human IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated
Rabbit anti-Human IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Mouse anti-Human IgG (Fc Region) antibody (Alexa Fluor 555)Animal-Free ELISA, FACS, WB, IF Multiclonal IgG2a Recombinant Antibody Alexa Fluor 555
Mouse anti-Human IgG (Fc Region) antibody (Alexa Fluor 488)Animal-Free ELISA, FACS, WB, IF Multiclonal IgG2a Recombinant Antibody Alexa Fluor 488
Mouse anti-Human IgG (Domain CH2), (Fc Region) antibody (HRP)ELISA, IHC (fro) Monoclonal MK 1 A6 IgG1 HRP
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Application Notes
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Suitable for Immunoblotting (1/1,000-1/5,000, Western or Dot blot), ELISA (1/10,000-1/40,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiringlot-to-lot consistency.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Reconstitution
- Restore with 1.0 mL of deionized water (or equivalent).
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Concentration
- 1.0 mg/mL
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Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as preservative.
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Preservative
- Gentamicin sulfate
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Precaution of Use
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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Handling Advice
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Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. -
Storage
- 4 °C/-20 °C
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Storage Comment
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- IgG
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Target Type
- Antibody
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Background
- Synonyms: Human Immunoglobulin G
Target
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