Donkey anti-Human IgG (Whole Molecule) Antibody (PE) - Preadsorbed

Catalog No. ABIN117825

Quick Overview for Donkey anti-Human IgG (Whole Molecule) Antibody (PE) - Preadsorbed (ABIN117825)

Target: IgG

Clonality: Polyclonal

Conjugate: PE

Application: Flow Cytometry (FACS), Immunomicroscopy (IM)

Product Details

Binding Specificity: Whole Molecule

Reactivity: Human

Host: Donkey

Purification: Preadsorption: Solid phase absorption

Immunogen: Human IgG whole molecule.

Application Details

Application Notes: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10^6 cellsin Flow Cytometry is approximately 1.0 g of antibody conjugate. Lesser amounts ofreagent may be sufficient for staining. As a general guideline dilutions of 1:50 to 1:200should be suitable for most applications.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Comment:

Absorption / Emission: 490 nm, 545 nm and 565 nm / 580 nm

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 0.5 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.02 % (w/v) Sodium Azide as preservative and 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Product is photosensitive and should be protected from light.
Avoid repeated freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

Target Details

Target: IgG

Target Type: Antibody

Background: Synonyms: Human Immunoglobulin G