Mouse anti-Rat IgG2c (Heavy Chain) Antibody

Catalog No. ABIN118449

Quick Overview for Mouse anti-Rat IgG2c (Heavy Chain) Antibody (ABIN118449)

Target: IgG2c

Clonality: Monoclonal

Application: Enzyme Immunoassay (EIA)

Clone: MARG2c-5

Isotype: IgG2a

Product Details

Binding Specificity: Heavy Chain

Reactivity: Rat

Host: Mouse

Purification: Affinity Chromatography

Immunogen: IgG2c (Rat strain LOU/c). Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Application Details

Application Notes: ELISA: 5 μg/mL, This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified rat IgG2c (SA024). Flow Cytometry: 1/200 - 1/1000, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

Target Details

Target: IgG2c

Target Type: Antibody

Background: Synonyms: Rat Immunoglobulin G2c