Mouse anti-Human IgE Antibody

Catalog No. ABIN120643

Quick Overview for Mouse anti-Human IgE Antibody (ABIN120643)

Target: IgE

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Clone: BE5

Isotype: IgG1

Product Details

Reactivity: Human

Host: Mouse

Purification: Precipitation methods and ion exchange chromatography

Purity: > 95 % (by SDS-PAGE)

Immunogen: Purified human IgE

Application Details

Application Notes: ELISA. Flow Cytometry: 5 - 10 μg/mL.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4-8 °C for up to one month or at -28 °C for longer.

Target Details

Target: IgE

Background: Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.Synonyms: Immunoglobulin E human

Molecular Weight: 180 kDa