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Mouse anti-Human IgM Antibody (APC)

FACS Monoclonal CH2 IgG1 APC
Catalog No. ABIN120645

Quick Overview for Mouse anti-Human IgM Antibody (APC) (ABIN120645)

Target

IgM

Clonality

  • 518
  • 109
Monoclonal

Conjugate

  • 122
  • 84
  • 76
  • 57
  • 33
  • 28
  • 27
  • 23
  • 13
  • 12
  • 11
  • 11
  • 11
  • 11
  • 11
  • 11
  • 11
  • 9
  • 9
  • 9
  • 9
  • 9
  • 7
  • 6
  • 5
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
APC

Application

  • 355
  • 316
  • 305
  • 237
  • 167
  • 115
  • 91
  • 91
  • 64
  • 64
  • 36
  • 35
  • 26
  • 22
  • 21
  • 15
  • 9
  • 6
  • 5
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS)

Clone

CH2

Isotype

IgG1
  • Reactivity

    • 223
    • 165
    • 89
    • 40
    • 30
    • 29
    • 28
    • 22
    • 17
    • 14
    • 13
    • 13
    • 13
    • 12
    • 12
    • 4
    • 2
    • 1
    Human

    Host

    • 353
    • 221
    • 97
    • 25
    • 14
    • 5
    • 4
    • 1
    • 1
    Mouse

    Purification

    Size-Exclusion Chromatography

    Immunogen

    Purified human IgM
  • Application Notes

    Suitable for Flow Cytometry (1/5 recommended starting point).

    Other applications not tested.
    Otimal dilutions are dependent on conditions and should be determined by the user.

    Comment

    Excitation/Emission wavelength: 650 nm/660 nm

    Restrictions

    For Research Use only
  • Concentration

    0.1 mg/mL

    Buffer

    PBS, 15 mM Sodium Azide, 0.2 % (w/v) high-grade Protease free BSA

    Preservative

    Sodium azide

    Precaution of Use

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Handling Advice

    Do not freeze!
    Protect conjugated products from light.

    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Should it contain a precipitate we recommend microcentrifugation before use.

    Storage

    4 °C

    Storage Comment

    Store the antibody undiluted at 2-8 °C.
  • Target

    IgM

    Target Type

    Antibody

    Background

    Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.Synonyms: Human Immunoglobulin M

    Molecular Weight

    900 kDa
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