Mouse anti-Chicken IgY Antibody - Preadsorbed

Catalog No. ABIN126817

Quick Overview for Mouse anti-Chicken IgY Antibody - Preadsorbed (ABIN126817)

Target: IgY

Clonality: Monoclonal

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: 9B4

Isotype: IgG1

Product Details

Reactivity: Chicken

Host: Mouse

Purification: Preadsorption: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography

Immunogen: Chicken egg yolk immunoglobulin (IgY) Molecular weight is 150 kDa

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL. Immunoprecipitation: 1 - 10 g.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2xPBS, 0.09 % Sodium Azide, PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Thaw aliquots at 30 °C. Thawed aliquots may be stored at 0 °C up to 3 months.

Target Details

Target: IgY

Alternative Name: IgG + IgY

Background: Synonyms: Chicken Immunoglobulin G, Chicken Immunoglobulin Y

Molecular Weight: 150 kDa