Goat anti-Mouse IgG, IgM Antibody - Preadsorbed

Catalog No. ABIN129739

Supplier Product No.: 610-101-115

Quick Overview for Goat anti-Mouse IgG, IgM Antibody - Preadsorbed (ABIN129739)

Target: IgG, IgM

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Supplier Product No.: 610-101-115

Supplier: Rockland

Purpose: Mouse IgG + IgM (H&L) Antibody Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against to Bv Hs & Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse IgM.  No reaction was observed against bovine, horse or human serum proteins.

Characteristics: Anti-Mouse IgG3 Antibody generated in goat detects reactivity to Mouse IgG3 (Gamma 3 chain).

Purification: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Mouse IgG and IgM whole molecules

Application Details

Application Notes: Application Note: Anti-Mouse IgG + IgM antibody has been tested by ELISA and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:2,000 - 1:10,000 Western Blot Dilution: 1:5,000 - 1:20,000 ELISA Dilution: 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

Target Details

Target: IgG, IgM

Alternative Name: IgG,IgM

Target Type: Antibody

Background: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG + IgM Antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.