Goat anti-Mouse IgA, IgG, IgM Antibody - Preadsorbed
Rockland 610-101-430
ELISA, IHC, WB, DB
Polyclonal
IgG
unconjugated
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- Target See all IgA, IgG, IgM products
- IgA, IgG, IgM
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Reactivity
- Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
- Supplier Product No.
- 610-101-430
- Supplier
- Rockland
- Purpose
- Mouse IgG IgA IgM (H&L) Pre-Adsorbed
- Cross-Reactivity (Details)
- Minimal crossreactivity against Bv, Hs, Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgA and Mouse IgM. No reaction was observed against bovine, horse or human serum proteins. This reagent is suitable for the detection of all mouse isotypes and chain combinations. ELISA was used to confirm specificity at less than 1 % of the target signal.
- Characteristics
- Anti-Mouse IgG IgA and IgM whole molecule antibody generated in goat detects specifically Mouse IgG IgA and IgM whole molecules. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Mouse IgG IgA IgM (H&L) Antibody is ideal for researchers in Cancer, Immunology, and Microbiology research.
- Purification
- Secondary Antibody was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by cross adsorption to remove unwanted specificities.
- Sterility
- Sterile filtered
- Immunogen
- Optional[Immunogen]: Mouse IgG IgA and IgM whole molecule
- Isotype
- IgG
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- Application Notes
- Application Note: Anti-Mouse IgG IgA IgM antibody has been tested by ELISA and Dot Blot, and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:50,000-1:90,000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.2 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- IgA, IgG, IgM
- Alternative Name
- IgG,IgA,IgM (IgA, IgG, IgM Products)
- Target Type
- Antibody
- Background
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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