Goat anti-Mouse IgG (Fc Region) Antibody - Preadsorbed

Catalog No. ABIN129747

Supplier Product No.: 610-1122

Product Details

Target: IgG

Binding Specificity: Fc Region

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Supplier Product No.: 610-1122

Supplier: Rockland

Purpose: Mouse IgG Fc Antibody Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bovine Horse and Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab')2 or Bovine, Horse and Human Serum Proteins.

Characteristics: Anti-Mouse IgG Antibody generated in goat detects reactivity to Mouse IgG.

Purification: Anti-Mouse IgG F(c) was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Mouse IgG F(c) fragment

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Mouse IgG F(c) antibody has been tested by ELISA and is suitable for ELISA, Western Blot and Immunohistochemistry applications. Antibody should be optimized by end user for specific reactive conditions. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:5,000 - 1:50,000 ELISA Dilution: 1:450,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.2 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.