Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (PE) - Preadsorbed

Catalog No. ABIN129765

Supplier Product No.: 611-108-122

Quick Overview for Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (PE) - Preadsorbed (ABIN129765)

Target: IgG

Clonality: Polyclonal

Conjugate: PE

Application: Western Blotting (WB), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Goat

Supplier Product No.: 611-108-122

Supplier: Rockland

Purpose: Rabbit IgG (H&L) Antibody Phycoerythrin conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

Characteristics: Anti-Rabbit IgG (H&L) Biotin Antibody generated in goat detects reactivity to Rabbit IgG.

Purification: Rabbit IgG (H&L) Antibody Phycoerythrin conjugated Pre-Adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, conjugation to phycoerythrin and subsequent purification, and extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-Rabbit IgG was produced by repeated immunization with rabbit IgG whole molecule in goat.

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Rabbit IgG (H&L) Phycoerythrin Antibody has been tested by western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate.  Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. Flow Cytometry Dilution: 1:100 - 1:250 IF Microscopy Dilution: 1:100 - 1:250 Other: FLOW 1:100 - 1:250

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.

Expiry Date: 12 months

References

Yasunaga, Ohtsubo, Ohno, Saeki, Kurogi, Tanaka-Okamoto, Ishizaki, Shirai, Mihara, Brock, Miyoshi, Takihara: "Scmh1 has E3 ubiquitin ligase activity for geminin and histone H2A and regulates geminin stability directly or indirectly via transcriptional repression of Hoxa9 and Hoxb4." in: Molecular and cellular biology, Vol. 33, Issue 4, pp. 644-60, (2013) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Anti-Rabbit IgG (H&L) phycoerythrin conjugated antibody generated in goat detects specifically rabbit IgG. This secondary phycoerythrin conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.