Rat anti-Mouse IgE (Heavy Chain) Antibody (HRP)

Catalog No. ABIN135037

Quick Overview for Rat anti-Mouse IgE (Heavy Chain) Antibody (HRP) (ABIN135037)

Target: IgE

Clonality: Monoclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC)

Clone: 23G3

Isotype: IgG1

Product Details

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Rat

Specificity: Reacts with the heavy chain of BALB/c mouse IgE as demonstrated by ELISA.

Purification: Affinity chromatography using phosphopeptide, cross-adsorbed with the corresponding non-phosphopeptide

Application Details

Application Notes: Working Dilution:
ELISA: 1:1,000-1:2,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 1.0 mL of stock solution in 50 % glycerol / 50 % PBS, pH 7.4.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C or long-term at -20 °C.

References

Amita, Rahul, Vivek, Chaudhari, Anurag, Mukul, Dwivedi: "Partial characterization of red gram (Cajanus cajan L. Millsp) polypeptides recognized by patients exhibiting rhinitis and bronchial asthma." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, (2010) (PubMed).

Target Details

Target: IgE