Mouse anti-Chicken IgM Antibody (PE)

Catalog No. ABIN135553

Quick Overview for Mouse anti-Chicken IgM Antibody (PE) (ABIN135553)

Target: IgM

Clonality: Monoclonal

Conjugate: PE

Application: ELISA, Flow Cytometry (FACS)

Clone: M-1

Isotype: IgG2b

Product Details

Reactivity: Chicken

Host: Mouse

Specificity: Chicken/Turkey IgM, Mr 820-950 kDa

Characteristics: Mouse Anti-Chicken IgM-PE

Purification: Immunoaffinity chromatography.

Immunogen: Affinity purified chicken Ig or isolated lymphocytes

Application Details

Application Notes:

• Applications: FC - Quality tested , ELISA - Quality tested FLISA - Quality tested IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Stim - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells APC, PE, SPRD, and AF647 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L FLISA FITC conjugate 1:100 - 1:400 PE, APC, and AF647 conjugates 1 g/mL ELISA BIOT conjugate 1:5,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use
Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Tepper, Jeschke, Böttcher, Schmidt, Davari, Müller, Kremmer, Hemmerich, Pfeil, Jungnickel: "PARP activation promotes nuclear AID accumulation in lymphoma cells." in: Oncotarget, Vol. 7, Issue 11, pp. 13197-208, (2017) (PubMed).

Fujita, Kitaura, Fujii: "A critical role of the Thy28-MYH9 axis in B cell-specific expression of the Pax5 gene in chicken B cells." in: PLoS ONE, Vol. 10, Issue 1, pp. e0116579, (2016) (PubMed).

Frankenberger, Davari, Fischer-Burkart, Böttcher, Tomi, Zimber-Strobl, Jungnickel: "Checkpoint kinase 1 negatively regulates somatic hypermutation." in: Nucleic acids research, (2014) (PubMed).

Target Details

Target: IgM

Target Type: Antibody