Mouse anti-Human IgG (Fc Region) Antibody (Biotin)

Catalog No. ABIN135621

Quick Overview for Mouse anti-Human IgG (Fc Region) Antibody (Biotin) (ABIN135621)

Target: IgG

Clonality: Monoclonal

Conjugate: Biotin

Application: ELISA, Flow Cytometry (FACS)

Clone: JDC-10

Isotype: IgG1

Product Details

Binding Specificity: Fc Region

Reactivity: Human

Host: Mouse

Specificity: Reacts with the Fc portion of the heavy chain of all subclasses of human IgG as demonstrated by ELISA.

Characteristics: Mouse Anti-Human IgG Fc-BIOT

Purification: Immunoaffinity chromatography

Immunogen: Purified human IgG1 myeloma protein

Application Details

Application Notes:

• Applications: ELISA - Quality tested , FLISA - Quality tested FC - Quality tested , IHC-PS - Reported in literature , WB - Reported in literature , Multiplex - Reported in literature
• Working Dilutions: ELISA AP conjugate 1:1,000 - 1:4,000 HRP conjugate 1:2,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA AF488 conjugate 1:200 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry Purified (UNLB) antibody 1 g/106 cells BIOT and AF488 conjugates 1 g/106 cells PE and AF647 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details

Target: IgG

Target Type: Antibody