Donkey anti-Chicken IgY Antibody (Agarose Beads)

Catalog No. ABIN1381671

Quick Overview for Donkey anti-Chicken IgY Antibody (Agarose Beads) (ABIN1381671)

Target: IgY

Conjugate: Agarose Beads

Application: Separation (Sep)

Product Details

Reactivity: Chicken

Host: Donkey

Purification: Antibodies were isolated from immune sera using affinity chromatography and conjugated to N- hydroxysuccinimide (NHS)-activated agarose beads. Beads were washed extensively after the blocking of the residual NHS sites.

The specific activity of the anti-IgY-agarose was determined by applying 2.7 mg IgY in 24 mL PBS (0.1 mg/mL) to 1.0 mL bed volume of agarose. Two mL aliquots were collected. The total amount of IgY that bound to the column was calculated. For this lot, 1.3 mg IgY bound per mL agarose.

Immunogen: A donkey was immunized with highly purified chicken IgY.

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Restrictions: For Research Use only

Handling

Format: Gel

Buffer: Phosphate buffered saline (PBS), pH 7.2

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Target Details

Target: IgY

Target Type: Antibody