Mouse anti-Human IgM Antibody

Catalog No. ABIN1607857

Supplier Product No.: 209-301-031

Quick Overview for Mouse anti-Human IgM Antibody (ABIN1607857)

Target: IgM

Clonality: Monoclonal

Application: ELISA, Western Blotting (WB)

Clone: 22G1-H8

Isotype: IgG2b

Product Details

Reactivity: Human

Host: Mouse

Supplier Product No.: 209-301-031

Supplier: Rockland

Purpose: Human IgM Fc5u Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgM, Human Serum and Human Fc5μ.  No reaction was observed against Human IgG, Human IgA, Human Fc, Human Fab or Human Fab2.

Characteristics: Anti-Human IgG F(c) generated in mouse detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .

Purification: Anti-Human IgM Fc5μ Antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Human IgM Fc5mu was produced by repeated immunization with Human IgM Fc5mu fragment in mice.

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Human IgM Fc5μ antibody has been tested by ELISA and Western Blot. This product is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Western Blot Dilution: 1:1000-1:2000 ELISA Dilution: 1:1000 - 1:10000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: IgM

Target Type: Antibody

Background: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Due to this large size, IgM is typically isolated to the serum. Anti-Human IgM Fc5μ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.