Goat anti-Mouse IgG Antibody (Colloidal Gold)

Catalog No. ABIN1720697

Quick Overview for Goat anti-Mouse IgG Antibody (Colloidal Gold) (ABIN1720697)

Target: IgG

Clonality: Polyclonal

Conjugate: Colloidal Gold

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoelectron Microscopy (IEM), Electron Microscopy (EM)

Product Details

Reactivity: Mouse

Host: Goat

Purpose: 5 nm Colloidal Gold Conjugated Goat Anti-mouse IgG

Specificity: This colloidal gold conjugated antibody is specific for mouse IgG and shows no cross-reactivity with mouse IgA, IgM and other animal species IgG proteins.

Purity: This antibody was purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for mouse IgG

Application Details

Application Notes: Add reagent grade BSA into 0.01 M PBS (PH7.2-7.6) or TBS buffer and make BSA at a concentration of 1%.
EM 1:20-50
IHC 1:50-100

Comment:

5 nm Colloidal Gold — Add 0.05% trisodium citrate and 0.05% Vitamin C into boiled 0.01% chloroauric acid which was reduced to gold granule solution through redox reaction. Conjugate colloidal gold to antibody after mensurating the optimal data. And through overspeed centrifugation and gradient delamination to density, the colloidal gold conjugated reagents were produced.

Restrictions: For Research Use only

Handling

Buffer: 0.1 mg of colloidal gold conjugated specific antibody, the diameter of colloidal gold is 5±1nm; 0.01M PBS; 1% BSA; 0.01% Thimerosal

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4℃ for frequent use; at -20℃ for at least one year

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody