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Goat anti-Human IgA (Chain alpha) Antibody (Biotin) - Preadsorbed

WB, IHC (fro), EIA Polyclonal Biotin
Catalog No. ABIN181418

Quick Overview for Goat anti-Human IgA (Chain alpha) Antibody (Biotin) - Preadsorbed (ABIN181418)

Target

IgA

Clonality

  • 307
  • 72
Polyclonal

Conjugate

  • 106
  • 54
  • 51
  • 39
  • 24
  • 18
  • 9
  • 6
  • 6
  • 6
  • 6
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Biotin

Application

  • 194
  • 144
  • 130
  • 107
  • 107
  • 104
  • 60
  • 47
  • 47
  • 34
  • 28
  • 25
  • 23
  • 18
  • 16
  • 16
  • 14
  • 7
  • 7
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
  • Binding Specificity

    • 69
    • 61
    • 37
    • 18
    • 15
    • 13
    • 8
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Chain alpha

    Reactivity

    • 188
    • 61
    • 45
    • 16
    • 15
    • 13
    • 13
    • 12
    • 12
    • 11
    • 9
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human

    Host

    • 200
    • 109
    • 58
    • 17
    • 14
    • 6
    • 2
    Goat

    Characteristics

    Molar Ratio: 10-20 BAC molecules per Goat IgG molecule

    Purification

    Preadsorption: Solid phase absorption

    Immunogen

    Human IgA (alpha chain)
  • Application Notes

    Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as otherantibodybased assays using streptavidin or avidin conjugates: ELISA: 1/60,000-1/600,000
    Western Blot: 1/2,000-1/10,000Immunohistochemistry: 1/1,000-1/5,000

    Other applications not tested.
    Otimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Reconstitution

    Restore with 1.0 mL of deionized water

    Concentration

    1.0 mg/mL

    Buffer

    0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, containg 10 mg/mL BSA (IgG and Protease free) and 0.01 % (w/v) Sodium Azide.

    Preservative

    Sodium azide

    Precaution of Use

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Handling Advice

    Avoid cycles of freezing and thawing.
    Centrifuge product if not completely clear after standing at room temperature.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store vial at 2-8 °C prior to restoration. For extended storage after reconstitution, mix product with glycerol to 50 % final, then aliquot contents and freeze at -28 °C or below. This product is stable for one month at 2-8 °C as an undiluted liquid.
  • Target

    IgA

    Target Type

    Antibody

    Background

    Synonyms: Human Immunoglobulin A
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