Guinea Pig anti-Rabbit IgG Antibody

Catalog No. ABIN1860986

Quick Overview for Guinea Pig anti-Rabbit IgG Antibody (ABIN1860986)

Target: IgG

Clonality: Polyclonal

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Isotype: IgG

Product Details

Reactivity: Rabbit

Host: Guinea Pig

Specificity: The antibody is a cavia polyclonal antibody raised against IgG. It has been selected for its ability to recognize IgG in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography

Immunogen: Native Immunoglobulin G (IgG)

Application Details

Application Notes:

• Western blotting: 0.5-2 μg/mL
• Immunocytochemistry in formalin fixed cells: 5-20 μg/mL
• Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL
• Immunohistochemistry in paraffin section: 5-20 μg/mL
• Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL
• Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody