Rabbit anti-Mouse IgG Antibody (PE)

Catalog No. ABIN1967823

Quick Overview for Rabbit anti-Mouse IgG Antibody (PE) (ABIN1967823)

Target: IgG

Clonality: Polyclonal

Conjugate: PE

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details

Reactivity: Mouse

Host: Rabbit

Specificity: The antibody shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.

Purification: Immunoaffinity purified

Immunogen: Anti-Mouse IgG subclass pan reactive Secondary Antibody was produced by repeated immunization with highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: Flo, IF (1:100 - 1:250), IHC

Usage: Anti-Mouse IgG subclass pan reactive Secondary Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA

Handling Advice: avoid freeze thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

Target Details

Target: IgG

Target Type: Antibody