Mouse anti-Human IgG4 (full length) Antibody

Catalog No. ABIN2127702

Quick Overview for Mouse anti-Human IgG4 (full length) Antibody (ABIN2127702)

Target: IgG4

Clonality: Monoclonal

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Isotype: IgG

Product Details

Binding Specificity: full length

Reactivity: Human

Host: Mouse

Characteristics: The antibody is a mouse monoclonal antibody raised against IgG4. It has been selected for its ability to recognize IgG4 in immunohistochemical staining and western blotting.

Purification: Affinity Chromatography

Immunogen: Full Length IgG4 of Human Plasma

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Store at 4 °C for frequent use. Stored at -20 °C to -80 °C in a manual defrost freezer for one year without detectable loss of activity.

Expiry Date: 12 months

Target Details

Target: IgG4

Alternative Name: Immunoglobulin G4 (IgG4)

Target Type: Antibody

Background: There are four IgG subclasses (IgG1, 2, 3, and 4) in humans, named in order of their abundance in serum. IgG4 antibodies will dominate the IgG response in schistosomiasis, lymphatic filariasis, and in patients after allergen immunotherapy. Unlike the other IgG subclasses, IgG4 does not activate complement. A combined IgA-IgG4 deficiency has been associated with recurrent pyogenic infections.