Goat anti-Human IgG (Fc Region) Antibody (HRP)

Catalog No. ABIN237483

Quick Overview for Goat anti-Human IgG (Fc Region) Antibody (HRP) (ABIN237483)

Target: IgG

Fragment: F(ab')2 fragment

Clonality: Polyclonal

Conjugate: HRP

Product Details

Binding Specificity: Fc Region

Reactivity: Human

Host: Goat

Cross-Reactivity (Details): Based on immunoelectrophoresis, the antibody reacts with the heavy chains on human IgG but not with the light chains on most human immunoglobulins. No antibody was detected against human IgM, IgA, or against non-immunoglobulin serum proteins. Has been passed through solid phase immunoadsorbent gels to remove antibodies which cross-react with bovine, horse, and mouse serum proteins, but antibodies may cross-react with immunoglobulins from other species. Minimal cross-reaction to Bovine, Horse, and Mouse Serum Proteins.

Characteristics: Goat anti Human IgG (Fc) F(ab' )2 Fragment, Goat antibody to Human Immunoglobulin G (IgG), Fc Fragment Specific Horseradish Peroxidase conjugated

Purification: Isolated from antisera by a combination of pepsin digestion and Immuno Affinity Chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.

Application Details

Comment:

Concentration (detection method): 0.8 mg/mL (prior to lyophilization)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute to 0.5 mL with distilled water.

Buffer: Lyophilized from 0.01 M Sodium Phosphate, 0.21 M Sodium Chloride, pH 7.6 containing 15 mg/mL BSA.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

Storage Comment: Store lyophilized product at 2-8 °C. After reconstitution, this product is stable for several weeks at 2-8 °C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50 % glycerol and store at -28 °C, with or without aliquoting.

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody