Mouse anti-Human IgG (Fc fragment) Antibody

Catalog No. ABIN2469245

Quick Overview for Mouse anti-Human IgG (Fc fragment) Antibody (ABIN2469245)

Target: IgG

Clonality: Monoclonal

Application: ELISA, Western Blotting (WB)

Clone: 4D2D9G8

Isotype: IgG1

Product Details

Binding Specificity: Fc fragment

Reactivity: Human

Host: Mouse

Characteristics: Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.

Purification: Antibody is purified by protein A affinity chromatography.

Immunogen: Chrompure Human IgG, Fc Fragment.

Application Details

Application Notes: Western Blot:1:200 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Buffer: Purified antibody in PBS containing 0.03 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: -20 °C

Storage Comment: IgG monoclonal antibody can be stored at -20 °C, stable for one year.

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Gene ID: 2212

UniProt: P12318