Mouse anti-Mouse IgDa Antibody (Biotin)
FACS, IF, IHC (fro), IP
Monoclonal
AMS 9-1
IgG2b kappa
Biotin
(2 references)-
- Target
- IgDa
- Reactivity
- Mouse
- Host
- Mouse
- Clonality
- Monoclonal
- Conjugate
- Biotin
- Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
- Brand
- BD Pharmingen™
- Characteristics
-
The AMS 9.1 antibody reacts specifically with the Fc portion of mouse IgD of Igh C[a] haplotype (eg, AKR, BALB/c, CBA, C3H/He, C58, DBA/1, DBA/2, NZB). It does not react with IgD of Igh-C[b] or Igh-C[e] haplotypes (eg, C57BL/6, C57BL/10, SJL, A). AMS 9.1 mAb is effective for detection of cell-surface Ig by immunofluorescent staining with flow cytometric analysis. It has been reported to stimulate B-cell proliferation.
BD Pharmingen™ Biotin Mouse Anti-Mouse IgD[a] - Biotin - Clone AMS 9.1 - Isotype Mouse IgG2b, κ - Reactivity Ms - 0.5 mg - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- BALB/c mouse splenocytes
- Clone
- AMS 9-1
- Isotype
- IgG2b kappa
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
-
-
: "In vitro and in vivo B lymphocyte-activating properties of monoclonal anti-delta antibodies. I. Determinants of B lymphocyte-activating properties." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 136, Issue 7, pp. 2382-92, (1986) (PubMed).
: "Allotypic specificities of murine IgD and IgM recognized by monoclonal antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 132, Issue 2, pp. 787-95, (1984) (PubMed).
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: "In vitro and in vivo B lymphocyte-activating properties of monoclonal anti-delta antibodies. I. Determinants of B lymphocyte-activating properties." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 136, Issue 7, pp. 2382-92, (1986) (PubMed).
-
- Target
- IgDa
- Alternative Name
- IgD[a]
- Background
- Synonyms: Igh-5a, Igh-5.4
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