Mouse anti-Mouse IgDa Antibody (Biotin)

Catalog No. ABIN2689624

Quick Overview for Mouse anti-Mouse IgDa Antibody (Biotin) (ABIN2689624)

Target: IgDa

Clonality: Monoclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Clone: AMS 9-1

Isotype: IgG2b kappa

Product Details

Reactivity: Mouse

Host: Mouse

Brand: BD Pharmingen™

Characteristics: The AMS 9.1 antibody reacts specifically with the Fc portion of mouse IgD of Igh C[a] haplotype (eg, AKR, BALB/c, CBA, C3H/He, C58, DBA/1, DBA/2, NZB). It does not react with IgD of Igh-C[b] or Igh-C[e] haplotypes (eg, C57BL/6, C57BL/10, SJL, A). AMS 9.1 mAb is effective for detection of cell-surface Ig by immunofluorescent staining with flow cytometric analysis. It has been reported to stimulate B-cell proliferation.

BD Pharmingen™ Biotin Mouse Anti-Mouse IgD[a] - Biotin - Clone AMS 9.1 - Isotype Mouse IgG2b, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: BALB/c mouse splenocytes

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Goroff, Stall, Mond, Finkelman: "In vitro and in vivo B lymphocyte-activating properties of monoclonal anti-delta antibodies. I. Determinants of B lymphocyte-activating properties." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 136, Issue 7, pp. 2382-92, (1986) (PubMed).

Stall, Loken: "Allotypic specificities of murine IgD and IgM recognized by monoclonal antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 132, Issue 2, pp. 787-95, (1984) (PubMed).

Target Details

Target: IgDa

Alternative Name: IgD[a]

Background: Synonyms: Igh-5a, Igh-5.4