Mouse anti-Mouse IgG1[a] Antibody (Biotin)

Catalog No. ABIN2689657

Quick Overview for Mouse anti-Mouse IgG1[a] Antibody (Biotin) (ABIN2689657)

Target: IgG1[a]

Clonality: Monoclonal

Conjugate: Biotin

Application: ELISA, Flow Cytometry (FACS)

Clone: 10-9

Isotype: IgG2a kappa

Product Details

Reactivity: Mouse

Host: Mouse

Characteristics: This antibody is specific for mouse IgG1 of the Igh-C[a] and the c, d, e, f, g, h, n, o, and p haplotypes (e.g. BALB/c, C58, A, AKR, CBA, C3H, DBA/1, DBA/2, NZB, SWR). It has been reported not to react with IgG1 of the Igh-C[b] haplotype (e.g. C57BL/6, C57BL/10, SJL).

BD Pharmingen™ Biotin Mouse Anti-Mouse IgG1[a] - Biotin - Clone 10.9 - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Huang, Huang, Lee: "Detection of immunoglobulin heavy chain IgG3 polymorphism in wild mice with xenogeneic monoclonal antibodies." in: Immunogenetics, Vol. 20, Issue 5, pp. 565-75, (1985) (PubMed).

Parsons, Oi, Huang, Herzenberg: "Structural characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies." in: Immunogenetics, Vol. 18, Issue 4, pp. 323-34, (1983) (PubMed).

Target Details

Target: IgG1[a]