Mouse anti-Mouse IgG2ab, Subclass Specific Antibody (Biotin)

Catalog No. ABIN2689675

Quick Overview for Mouse anti-Mouse IgG2ab, Subclass Specific Antibody (Biotin) (ABIN2689675)

Target: IgG2ab (IgG2ab, Subclass Specific)

Clonality: Monoclonal

Conjugate: Biotin

Application: ELISA, Flow Cytometry (FACS)

Clone: 5-7

Isotype: IgG3 kappa

Product Details

Reactivity: Mouse

Host: Mouse

Characteristics: This antibody reacts specifically with mouse IgG2a of Igh-C [b] haplotype (e.g., C57BL/6, C57BL/10, NOD, SJL). It has been reported not to react with IgG2a of the a, c, d, e, f, g, h, j, n, o, or p haplotypes (e.g., A, AKR, BALB/c, C3H/He, C58, CBA, DBA/1, DBA/2, NZB, SWR). Molecular genetic analyses suggest that the Igh-1 [b] allele, which encodes IgG2a [b], is derived from a locus found in several wild mouse subspecies, but not domestic mice, which encodes the IgG2c isotype.

BD Pharmingen™ Biotin Mouse Anti-Mouse IgG2a [b] - Biotin - Clone 5.7 - Isotype Mouse IgG3, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Not Reported

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Martin, Silva, Lew: "The Igh-1 sequence of the non-obese diabetic (NOD) mouse assigns it to the IgG2c isotype." in: Immunogenetics, Vol. 46, Issue 2, pp. 167-8, (1997) (PubMed).

Morgado, Cam, Gris-Liebe, Cazenave, Jouvin-Marche: "Further evidence that BALB/c and C57BL/6 gamma 2a genes originate from two distinct isotypes." in: The EMBO journal, Vol. 8, Issue 11, pp. 3245-51, (1989) (PubMed).

Huang, Parsons, Oi, Huang, Herzenberg: "Genetic characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies." in: Immunogenetics, Vol. 18, Issue 4, pp. 311-21, (1983) (PubMed).

Parsons, Oi, Huang, Herzenberg: "Structural characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies." in: Immunogenetics, Vol. 18, Issue 4, pp. 323-34, (1983) (PubMed).

Target Details

Target: IgG2ab (IgG2ab, Subclass Specific)

Alternative Name: IgG2a [b]