Mouse anti-Mouse IgG2a[a] Antibody (Biotin)

Catalog No. ABIN2689677

Quick Overview for Mouse anti-Mouse IgG2a[a] Antibody (Biotin) (ABIN2689677)

Target: IgG2a[a]

Clonality: Monoclonal

Conjugate: Biotin

Application: ELISA, Flow Cytometry (FACS)

Clone: 8-3

Isotype: IgG2a kappa

Product Details

Reactivity: Mouse

Host: Mouse

Characteristics: The 8.3 antibody reacts specifically with mouse IgG2a of Igh-Ca and the d, e, f, g, h, j, n, and o haplotypes (eg, BALB/c, C58, and A, AKR, CBA, C3H/He, NZB). It does not react with IgG2a of Igh-Cb nor the c and p haplotypes (eg, C57BL/6, C57BL/10, SJL, DBA/1, DBA/2, SWR).

BD Pharmingen™ Biotin Mouse Anti-Mouse IgG2a[a] - Biotin - Clone 8.3 - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Huang, Parsons, Oi, Huang, Herzenberg: "Genetic characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies." in: Immunogenetics, Vol. 18, Issue 4, pp. 311-21, (1983) (PubMed).

Parsons, Oi, Huang, Herzenberg: "Structural characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies." in: Immunogenetics, Vol. 18, Issue 4, pp. 323-34, (1983) (PubMed).

Target Details

Target: IgG2a[a]