Mouse anti-Mouse IgMa Antibody (Biotin)

Catalog No. ABIN2689712

Quick Overview for Mouse anti-Mouse IgMa Antibody (Biotin) (ABIN2689712)

Target: IgMa

Clonality: Monoclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: DS

Isotype: IgG1 kappa

Product Details

Reactivity: Mouse

Host: Mouse

Brand: BD Pharmingen™

Characteristics: The DS-1 antibody reacts specifically with mouse IgM of Igh-C[a] and related haplotypes (e.g., BALB/c, C58, CBA, C3H/Bi, C3H/He, DBA/1, DBA/2). It does not react with IgM of Igh-C[b], Igh-C[e], or related hapltypes (e.g., C57BL, SJL, A, AKR, NZB). DS-1 antibody does not react with free μ heavy chain in vitro or in the cytoplasm of pre-B lymphocytes, which lack Ig light chain. It has not been shown to stimulate B-cell proliferation.

BD Pharmingen™ Biotin Mouse Anti-Mouse IgM[a] - Biotin - Clone DS-1 - Isotype Mouse IgG1, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse NMRI Monoclonal Antibody TC31

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Garside, Ingulli, Merica, Johnson, Noelle, Jenkins: "Visualization of specific B and T lymphocyte interactions in the lymph node." in: Science (New York, N.Y.), Vol. 281, Issue 5373, pp. 96-9, (1998) (PubMed).

Target Details

Target: IgMa

Alternative Name: IgM[a]