Mouse anti-Rabbit IgG (Whole Molecule) Antibody (HRP)

Catalog No. ABIN300865

Quick Overview for Mouse anti-Rabbit IgG (Whole Molecule) Antibody (HRP) (ABIN300865)

Target: IgG

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Binding Specificity: Whole Molecule

Reactivity: Rabbit

Host: Mouse

Specificity: Anti-Peroxidase, anti-Mouse Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against or Goat, Human and Mouse Serum Proteins

Purification: Immunoaffinity purified

Immunogen: Rabbit IgG whole molecule.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA (1:8000 - 1:32000), IHC, WB

Usage: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Rabbit IgG in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Rabbit

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % Gentamicin sulfate.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details

Target: IgG

Target Type: Antibody