Rabbit anti-Mouse IgG Antibody (Biotin)

Catalog No. ABIN301149

Quick Overview for Rabbit anti-Mouse IgG Antibody (Biotin) (ABIN301149)

Target: IgG

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Product Details

Reactivity: Mouse

Host: Rabbit

Specificity: Anti-biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against other Mouse heavy or light chain proteins

Purification: Immunoaffinity purified

Immunogen: Mouse IgG gamma chain.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:50000), IF, IHC, WB

Usage: Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Prior to reconstitution, 4°C. Following reconstitution store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: IgG

Target Type: Antibody