Donkey anti-Sheep IgG (Whole Molecule) Antibody (DyLight 488)

Catalog No. ABIN3045811

Quick Overview for Donkey anti-Sheep IgG (Whole Molecule) Antibody (DyLight 488) (ABIN3045811)

Target: IgG

Conjugate: DyLight 488

Application: Flow Cytometry (FACS), Immunomicroscopy (IM)

Product Details

Binding Specificity: Whole Molecule

Reactivity: Sheep

Host: Donkey

Specificity: Based on IEP, this antibody reacts with:
heavy (γ) chains on sheep IgG
light chains on all sheep immunoglobulins

Cross-Reactivity (Details): Based on IEP, no reactivity is observed to:
non-immunoglobulin sheep serum proteins

Characteristics: Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Purification: Affinity purified using solid phase Sheep IgG

Purity: > 95 % based on SDS-PAGE

Immunogen: Purified Sheep IgG, whole molecule

Application Details

Application Notes: This conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator.
Suggested starting dilution(s): • 1:20 - 1:2,000 for most applications

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Concentration: 1 mg/mL

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store freeze-dried powder at 2-8 °C.
Shelf Life: Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.

Target Details

Target: IgG

Target Type: Antibody