Rabbit anti-Cow IgG (Whole Molecule) Antibody (HRP)

Catalog No. ABIN3045918

Quick Overview for Rabbit anti-Cow IgG (Whole Molecule) Antibody (HRP) (ABIN3045918)

Target: IgG

Conjugate: HRP

Application: Immunoassay (IA)

Product Details

Binding Specificity: Whole Molecule

Reactivity: Cow

Host: Rabbit

Specificity: Based on IEP, this antibody reacts with:
heavy (γ) chains on bovine IgG
light chains on all bovine immunoglobulins

Cross-Reactivity (Details): Based on IEP, no reactivity is observed to:
non-immunoglobulin bovine serum immunoglobulins

Characteristics: Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Purification: Affinity purified using solid phase Bovine IgG (H&L)

Purity: > 95 % based on SDS-PAGE

Immunogen: Purified Bovine IgG, whole molecule

Application Details

Application Notes: This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator.
Suggested starting dilution(s): • 1:500-1:5,000 for Immunohistochemistry • 1:200-1:5000 for ELISA/Western blot

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Concentration: 1.0 mg/mL

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
0.1 % (v/v) Kathon CG

Preservative: Kathon CG

Storage: 4 °C

Storage Comment: Store freeze-dried powder at 2-8 °C.
Shelf Life: Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.

Target Details

Target: IgG

Target Type: Antibody